1. What is DNA Molarity?

DNA molarity represents the concentration of DNA molecules in solution, expressed in picomoles per microliter (pmol/μL). It's essential for molecular biology applications where precise amounts of DNA are required.

2. How Does the Calculator Work?

The calculator uses the DNA molarity formula:

Where:

• \( M \) — DNA molarity (pmol/μL)
• \( C \) — DNA concentration (μg/μL)
• \( L \) — DNA length (base pairs)
• 660 — Average molecular weight of a DNA base pair (g/mol)
• 10^6 — Conversion factor from μg to pg and mol to pmol

Explanation: The equation converts mass concentration to molar concentration by accounting for the molecular weight of DNA and the length of your specific fragment.

3. Importance of DNA Molarity Calculation

Details: Accurate molarity calculations are crucial for PCR, sequencing, cloning, and other molecular biology techniques where precise DNA amounts are needed for optimal results.

4. Using the Calculator

Tips: Enter DNA concentration in μg/μL and length in base pairs. Both values must be positive numbers. The calculator will output molarity in pmol/μL.

5. Frequently Asked Questions (FAQ)

Q1: Why is 660 used in the formula?
A: 660 g/mol is the average molecular weight of one DNA base pair, accounting for different nucleotide compositions.

Q2: Does this work for single-stranded DNA?
A: No, for single-stranded DNA use 330 g/mol as the average molecular weight per nucleotide.

Q3: What's a typical molarity range for PCR?
A: Most PCR reactions use 0.1-1 pmol of template DNA per 50 μL reaction.

Q4: How accurate is this calculation?
A: It provides a good estimate but actual molecular weight may vary slightly based on nucleotide composition.

Q5: Can I use ng/μL instead of μg/μL?
A: Yes, but you'll need to adjust the formula by dividing by 1000 or convert your units first.