1. What is DNA Copy Number and Dilution?

The DNA copy number and dilution calculation helps determine the concentration of DNA molecules after a dilution process. This is essential for accurate molecular biology experiments and quantitative PCR applications.

2. How Does the Calculator Work?

The calculator uses the simple dilution formula:

Where:

• \( CN_d \) — Diluted copy number (dimensionless)
• \( CN \) — Original copy number (dimensionless)
• \( D \) — Dilution ratio (dimensionless)

Explanation: The equation shows the direct relationship between the original copy number and the dilution factor.

3. Importance of Copy Number Calculation

Details: Accurate copy number calculation after dilution is crucial for preparing samples with known concentrations for experiments like qPCR, sequencing, and other molecular biology techniques.

4. Using the Calculator

Tips: Enter the original copy number and dilution ratio. Both values must be positive numbers. The result will be the copy number after dilution.

5. Frequently Asked Questions (FAQ)

Q1: What units should I use for copy number?
A: Copy number is dimensionless, but it should represent the absolute number of DNA molecules in your sample.

Q2: How do I determine my original copy number?
A: Original copy number can be determined using spectrophotometry, fluorometry, or digital PCR methods.

Q3: What if my dilution ratio is less than 1?
A: A dilution ratio less than 1 actually represents concentration, not dilution. This calculator assumes proper dilution (D > 1).

Q4: Can I use this for serial dilutions?
A: For serial dilutions, you would need to apply this calculation sequentially for each dilution step.

Q5: How precise is this calculation?
A: The calculation is mathematically precise, but actual experimental results may vary due to pipetting errors and other factors.