1. What is DNA Molar Concentration?

DNA molar concentration refers to the number of moles of DNA per liter of solution (μM). It's essential for molecular biology experiments where precise DNA amounts are required for reactions like PCR, cloning, and sequencing.

2. How Does the Calculator Work?

The calculator uses the DNA molar concentration formula:

Where:

• \( M \) — Molar concentration (μM)
• \( C \) — DNA concentration (μg/mL)
• \( MW \) — Molecular weight (g/mol)

Explanation: The formula converts mass concentration (μg/mL) to molar concentration (μM) by accounting for the molecular weight of the DNA.

3. Importance of Molar Concentration

Details: Accurate molar concentration is crucial for experiments requiring precise DNA molecule counts, ensuring consistent results in quantitative applications.

4. Using the Calculator

Tips: Enter DNA concentration in μg/mL and molecular weight in g/mol. Both values must be positive numbers.

5. Frequently Asked Questions (FAQ)

Q1: How do I determine the molecular weight of my DNA?
A: For double-stranded DNA, MW ≈ (number of base pairs) × 650 g/mol/bp. For single-stranded DNA or oligos, use exact sequence calculations.

Q2: Why convert to molar concentration?
A: Molar concentration tells you how many DNA molecules are present, which is more meaningful than mass concentration for many applications.

Q3: What's a typical DNA concentration range?
A: Purified DNA samples typically range from 10-1000 ng/μL (10-1000 μg/mL), depending on the preparation method.

Q4: Does this work for RNA too?
A: Yes, the same formula applies to RNA concentration calculations.

Q5: How accurate is this calculation?
A: The calculation is mathematically precise, but accuracy depends on your input measurements (concentration and MW).