DNA to mRNA Conversion:
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The process of converting DNA to mRNA is called transcription. During transcription, the DNA sequence is copied into messenger RNA (mRNA), with thymine (T) bases replaced by uracil (U) bases.
The conversion follows this simple rule:
Where:
Explanation: In RNA, uracil (U) replaces thymine (T) found in DNA. All other bases remain the same.
Details: mRNA carries genetic information from DNA to ribosomes, where it serves as a template for protein synthesis during translation.
Tips: Enter a valid DNA sequence (only A, T, C, G characters). The sequence is case-insensitive and will be converted to uppercase.
Q1: Why does RNA use U instead of T?
A: Uracil is used in RNA because it's more stable for single-stranded molecules and requires less energy to produce than thymine.
Q2: Does the DNA sequence need to start with ATG?
A: No, this calculator converts any valid DNA sequence. ATG is the start codon for protein coding regions.
Q3: Should I include non-coding regions?
A: You can include any DNA sequence, but only coding regions (exons) will be translated into proteins.
Q4: What about the complementary strand?
A: This calculator assumes you're entering the template strand. The coding strand would need to be complemented first.
Q5: How does this relate to cDNA?
A: cDNA is synthesized from mRNA using reverse transcriptase, which would convert U back to T.